3 edition of Determination of hemoglobin adducts following acrylamide exposure found in the catalog.
Determination of hemoglobin adducts following acrylamide exposure
by U.S. Environmental Protection Agency, Environmental Monitoring Systems Laboratory, National Technical Information Service, [distributor in Las Vegas, NV, Springfield, VA
Written in English
|Statement||L.G. Costa and C.J. Calleman.|
|Contributions||Calleman, C. J., Environmental Monitoring Systems Laboratory (Las Vegas, Nev.)|
|The Physical Object|
The concentration of acrylamide bound to the N‐terminal amino acid in hemoglobin is strongly correlated to the exposure of acrylamide, 3, while its glycidamide analog correlates to glycidamide DNA adducts, 37 and is considered a biomarker for the genotoxic dose reflecting the individual ability to activate acrylamide to glycidamide. Abstract. Acrylamide (AM), which is used to manufacture polymers, is carcinogenic and a reproductive and neurological toxicant. The objective of this study was to compare the metabolism of AM administered orally (po), dermally, intraperitoneally (ip), or by inhalation, and to measure the hemoglobin adducts .
Smoking is an important source of acrylamide exposure in the general population. We assessed the relationship between hemoglobin adducts of acrylamide (HbAA) and glycidamide (HbGA) as biomarkers of acrylamide exposure and plasma cotinine (PC) as biomarkers of tobacco smoke exposure in 94 men and 67 women. The median (5thth percentile) biomarker concentrations . Determination of haemoglobin adducts of acrylamide and glycidamide in smoking and non-smoking persons of the general population. International Journal of Hygiene and Environmental Health , , DOI: /
Dietary acrylamide exposure assessment using FFQs has been criticized due to its low correlation with hemoglobin adducts of exposure in many epidemiologic studies (correlation range: –; ref. 19); however, this weakness was avoided because our exposure data were based on hemoglobin adducts . The relationship between the acrylamide and glycidamide adducts on the N-terminal valine of hemoglobin is proportional in man and rat. Comparison of free acrylamide in plasma, valine adducts on haemoglobin, and urinary S -(2-carboxyethyl)cysteine indicates that the rate of elimination of acrylamide is at least five times lower in man than in.
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The complete report, entitled "Determination of Hemoglobin Adducts Following Acrylamide Exposure," (Order No. PB; Cost: $; subject to change) will be available only from: National Technical Information Service Port Royal Road Springfield, VA Telephone: The EPA Project Officer can be contacted at.
Occupational exposure to acrylamide was already studied for many years by measurement of reaction products (adducts) to hemoglobin. One of the advantages of the determination of adducts from small molecules such as acrylamide and glycidamide, with the N-terminal valine of hemoglobin, lies in the chemical stability of the adducts reaction between acrylamide Cited by: Determination of hemoglobin adducts in humans occupationally exposed to acrylamide.
Bergmark E(1), Calleman CJ, He F, Costa LG. Author information: (1)Department of Environmental Health, University of Washington, Seattle Hemoglobin (Hb) adduct determinations were used to monitor occupational exposure to acrylamide (AA) and acrylonitrile Cited by: Hemoglobin (Hb) adduct determinations were used to monitor occupational exposure to acrylamide (AA) and acrylonitrile (AN).
Forty-one workers in a factory in the People′s Republic of China who were involved in the synthesis of AA by catalytic hydration of AN and the manufacturing of polyacrylamides were by: Acrylamide is a neurotoxic and carcinogenic organic compound that is able to bind to several biomolecules and form adducts, through nucleophilic addition and in vivo by the Maillard Reaction, interfering with the biological functions of these molecules.
Hemoglobin is one of the most abundant intracellular blood proteins, and thus it is of high interest to understand whether the binding of Author: André G.
Favinha, Daniela S. Barreiro, Joana N. Martins, Philip O'Toole, Sofia R. Pauleta. The determination of biomarkers of acrylamide exposure in humans from general French population by measurement of hemoglogin adduct levels of acrylamide (AA) and glycidamide (GA) is presented.
The analytical procedure included modified Edman degradation and LC–ESI-MS/MS analysis of the final derivatives using deuterated internal standards. Hemoglobin adducts of acrylamide were comprehensively profiled.
• Enantiomer pair of hemoglobin adducts of glycidamide was firstly identified. • Hemoglobin adducts were rapidly quantified within only 12 min per run. • This method was used for internal exposure evaluation of acrylamide in humans. We measured acrylamide exposure using red blood cell acrylamide and glycidamide hemoglobin adducts among women in two large prospective cohorts: the Nurses’ Health Study and Nurses’ Health Study II.
Between blood collection andwe identified incident cases of epithelial ovarian cancer, matching two controls per case. DETERMINATION OF HEMOGLOBIN ADDUCTS FOLLOWING ACRYLAMIDE EXPOSURE Lucio G. Costa, Ph.D., Principal Investigator Carl J.
Calleman, Ph.D., Co-Principal Investigator Department of Environmental Health, SC University of Washington Seattle, WA Cooperative Agreement CR Project Officer: Charles H. Nauman, Ph.D., M.P.H. Exposure Assessment Research. The knowledge about fetal exposure to acrylamide/glycidamide from the maternal exposure through food is limited.
Acrylamide, glycidamide, and ethylene oxide are electrophiles and form adducts with hemoglobin (Hb), which could be used for in vivo dose measurement. In this study, a method for analysis of Hb adducts by liquid chromatography–mass spectrometry, the adduct FIRE procedure.
Hemoglobin adducts of acrylamide and its primary metabolite, glycidamide are used as biomarkers of acrylamide exposure. Several methods for analyzing these biomarkers in blood have been described previously. These methods were developed to analyze small numbers of samples, not the high sample throughput that is needed in population screening.
To examine the coherence of estimated intakes of acrylamide (AA) from foods, with hemoglobin (Hb) AA adduct levels, an objective marker of environmental AA exposure.
A cross-sectional study. The. The variation in dietary exposure to acrylamide (AA) has been studied through measurement of hemoglobin adduct levels from AA, as a measurement of internal dose, in a sample from the blood bank of the Malmö Diet and Cancer cohort (n=28,).
Role of CYP2E1 in the epoxidation of acrylamide to glycidamide and formation of DNA and hemoglobin adducts. Toxicol. Sci. ; – Hagmar L, Wirfalt E, Paulsson B, Tornqvist M. Differences in hemoglobin adduct levels of acrylamide in the general population with respect to dietary intake, smoking habits and gender.
Mutat. Res. The variation in dietary exposure to acrylamide (AA) has been studied through measurement of hemoglobin adduct levels from AA, as a measurement of internal dose, in.
Hemoglobin adduct levels were elevated after exposures to styrene, MDI, MDA, 1, 3-butadiene, ethylene oxide, acrylamide and acrylonitrile. Moreover, hemoglobin adducts of butadiene, ethylene oxide, acrylamide and acrylonitrile have several useful advantages. For example, the hemoglobin adduct of 1,3-butadiene is an even more useful biomarker of.
However, accurate acrylamide exposure assessment in epidemiological studies based on questionnaires has been difficult, and more direct measures of molecular markers, such as hemoglobin adduct. Hemoglobin (Hb) adducts, formed by the neurotoxic agent acrylamide (AA) and its genotoxic metabolite glycidamide (GA), were measured in the rat by means of a method for simultaneous determination of the adducts formed to cysteine.
A novel, nonlinear dosimetric model was developed to describe Hb adduct formation. Toxicity of acrylamide and evaluation of its exposure in baby foods - Volume 23 Issue 2 - Pınar Erkekoğlu, Terken Baydar This article has been cited by the following publications.
This list is generated based on data provided by CrossRef. Determination of hemoglobin adducts in humans occupationally exposed to acrylamide. The aim of this study was to determine the relationship between the oxidative and reductive metabolic pathways of acrylamide (AA) in the nonsmoking general population.
For the first time both the blood protein adducts and the urinary metabolites of AA and glycidamide (GA) were quantified in an especially designed study group with even distribution of age and gender.
The hemoglobin adducts N. products have higher acrylamide exposure than those not smoking (Vesper ). Exposure to second hand smoke seems to have a small but significant effect on hemoglobin adduct levels in non-smokers (Vesper ). Analytical methods measuring hemoglobin adducts of acrylamide determine the adducts.Acrylamide is a chemical which is extensively used in research laboratories for the preparation of polyacrylamide gels for electrophoresis (PAGE).
Blood samples were collected from laboratory personnel who were working with PAGE, from smokers, and from nonsmokers. Hemoglobin adducts of acrylamide, acrylonitrile, and ethylene oxide were determined using the modified Edman degradation .Both acrylamide and glycidamide form bonds with the N-terminal valine of globin chains in hemoglobin.
These hemoglobin adducts would be expected to provide a time-integrated measure of exposure because the half-life of red blood cells is approximately days. A major advantage of comparing FFQ acrylamide intake to Hb adducts is that.